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KMID : 0545120020120060929
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 6 p.929 ~ p.935
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Microbial Community Analysis of 5-Stage Biological Nutrient Removal Process with Step Feed System
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PARK, JONG BOK
LEE, HAN WOONG/LEE, SOO-YOUN/LEE, JUNG OK/BANG, IEL SOO/CHOI, EUI SO/PARK, DOO HYUN
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Abstract
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The 5-stage biological nutrient removal (BNR) process with step feed system showed a very stable organic carbon and nutrient removal efficiency (87% COD, 79% nitrogen, and 87% phosphorus) for an operation period of 2 years. In each stage at the pilot plant, microbial communities, which are important in removing nitrogen and phosphorus, were investigated using fluorescence in-situ hybridization (FISH) and 16S rDNA characterization. All tanks of 5-stage sludge had a similar composition of bacterial communities. The total cell numbers of each reactor were found to be around 2.36-2.83×10^9 cells/ml. About 56.5-62.0% of total 4, 6-diamidino-2-phenylindol (DAPI) cells were hybridized to the bacterial-specific probe EUB388. Members of β-proteobacteria were the most abundant proteobacterial group, accounting for up to 20.6-26.7%. The high G+C Gram-positive bacterial group and Cytophaga-Flexibacter cluster counts were also found to be relatively high. The beta subclass proteobacteria did not accumulate a large amount of polyphosphate. The proportion of phosphorus-accumulating organisms (PAOs) on the total population of the sludge was almost 50% in anoxic-1 tank. The high G+C Gram-positive bacteria and Cytophaga-Flexilacter cluster indicate a key role of denitrifying phosphorus-accumulating organisms (dPAOs). Both groups might be correlated with some other subclass of proteobacteria for enhancing nitrogen and phosphorus removal in this process.
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KEYWORD
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